The use of recombinant proteins in research, diagnostics, and therapeutic applications has grown enormously in recent years. These proteins are crucial for many aspects of life processes, including protein folding, hormone production, cellular signaling, and blood clotting. Therapeutic proteins are used to treat conditions like diabetes, cancer, infectious diseases, hemophilia, and anemia.
Producing these proteins requires a number of complex molecular engineering steps, starting with cloning the protein-coding sequences into an expression vector and introducing it into a host cell. The host cell then uses its protein synthesis machinery to produce the desired recombinant protein. The choice of the expression system used for protein production is critical because it determines the quality and quantity of the recombinant protein produced. A suitable expression vector is also important because it inserts the gene into the host cell in a way that is compatible with the target protein’s protein-folding and aggregation characteristics.
Custom Protein Synthesis: Precision for Complex Applications
Using an appropriate expression system optimizes protein production and purification, increasing the yield of soluble proteins. The recombinant protein can then be conjugated with fluorophores, biotin, PEG, or other chemical labels, for use in an experimental assay. The recombinant protein can also be produced under stringent Good Manufacturing Practice (GMP) and Animal-Free quality requirements, for use as an ex vivo cell therapy reagent or ancillary protein.
Choosing a protein production service provider with experience in custom protein synthesis will help you avoid delays and costs. A reliable provider will have a rapid turnaround time and responsive customer support, and can provide technical expertise for your project. In addition, the company should be in compliance with all applicable regulations and guidelines.